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Molecular Technologist- Pathology

Boston Childrens Hospital - Boston, MA

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Job Description

Job Posting DescriptionPosition Summary/ Department Summary:Working independently under minimal supervision, perform complex technical work involving molecular analysis of human biological specimens. Responsibilities include multi-step workflows requiring precise manual manipulation and pipetting, careful monitoring and control of processes, data evaluation, and a high level of accuracy and attention to detail.Key Responsibilities:• Evaluate and accession specimens received by the department and organize workflow to optimize efficiency and turnaround time.• Perform extraction of DNA, RNA, and TNA from a variety of specimen types using both automated and manual workflows, including whole blood, bone marrow, bone marrow smears, FFPE, fresh or frozen tissues, buffy coat, and cell pellets.• Perform molecular assays, including real-time RT-qPCR, ddPCR, Real-Time qPCR, hybridization, and next-generation sequencing (NGS).• Evaluate results to identify potential issues such as interference, inhibition, or assay failure during extraction, amplification, or sequencing.• Perform quality control, verification, validation, and other quality assurance activities to ensure optimal assay performance while maintaining strict adherence to SOPs and best practices.• Conduct and record routine maintenance (daily, weekly, monthly, semi-annual, and annual) of laboratory instruments and troubleshoot equipment issues as needed.• Manage inventory of supplies and reagents and communicate ordering needs.• Operate complex laboratory instrumentation and associated software, including laboratory information systems (LIS).• Maintain accurate records of test results and ensure proper data archiving.• Perform proficiency testing to maintain compliance with regulatory agencies.• Assist in the development, review, and maintenance of standard operating procedures (SOPs).• Maintain competency by reviewing and acknowledging SOPs on a regular basis.• Adhere to laboratory policies and procedures, including attendance and time reporting.Minimum QualificationsEducation:Bachelor's degree in Biology or a related field required, with training in molecular biology techniques and/or experience in clinical molecular diagnostics.Experience:• Minimum of 2 years of relevant experience is required.• Experience with in-house molecular diagnostic assay design and development, including design of primers and probes, assay validation, quality control, quality assurance, and/or troubleshooting, is desired.Ability to follow standard protocols and independently perform routine molecular testing in a clinical laboratory setting.Experience working with formalin-fixed, paraffin-embedded (FFPE) samples is preferred.Experience with amplicon-based targeted sequencing, bead-based workflows, and multichannel pipetting is preferred.Licensure/ Certifications:Molecular Biology MB ASCP Certification preferred or eligibility for certification in Molecular Biology MB ASCP is preferred.

Created: 2026-04-16

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