Conduct multi-disciplinary field work to study the coastal methane cycle.Extract DNA and RNA from mangrove tissue and mangrove soil.Quantify populations of methane-cycling microorganisms using real-time PCR.Perform metabarcoding and DNA sequence analyses of methane-cycling microbial populations in mangrove tissue and mangrove soilAnalysis of stable carbon isotopic compositions of methane and other carbon compounds present in environmental samples.