Protein Science Principal Research Associate

Job DescriptionProtein Science Principal Research Associate (Protein Engineering, Protein Production)General/Position SummaryThe Protein Sciences Principal Research Associate provides laboratory support in advancement of Protein Engineering pipeline with conducting cloning workflows and plasmid preparation. They work closely with protein engineers to provide support with yeast transformation, yeast cultures and characterization by flow cytometry. They provide support for mammalian expressions and high-throughput purification and biophysical characterization of proteins as needed.Key Duties & ResponsibilitiesExecute and troubleshoot molecular cloning workflows spanning plasmid assembly to plasmid preparation at mini- and midi- scalesPerform small-scale yeast transformations and culturingSupport characterization of yeast displayed proteins by flow cytometrySupport large-scale yeast transformations with DNA librariesSupport lab operations and equipment purchasing if neededPerform high-throughput purification of proteins and protein-analytics using UV-Vis Spectroscopy, analytical SEC (UPLC-HPLC through Empower software), endotoxin tests, SDS-PAGE, and western blottingManage protein inventory in the protein database and Protein Science freezersProvide protein dilutions and protein aliquots to immunology group and pharmacology group as neededDeliver verbal and presentation updates on cloning, expression, purification and analytics to the Protein Science Team.Ensure that a complete and timely record of experiments, constructs, plasmid preps is maintained in both the protein database and electronic lab notebooksRequired Education LevelMaster's Degree (or equivalent degree) in cellular biology, biochemistry, biophysics, or bio/chemical engineering and 3+ years of relevant industry experience, orBachelor's Degree (or equivalent degree) in cellular biology, biochemistry, biophysics, or bio/chemical engineering and 6 + years of relevant industry experienceRequired Knowledge/SkillsExperience using electronic lab notebooks, cloning software such as snap geneExperience in working in a highly collaborative laboratory which is timeline-driven and result orientated team is a mustExperience in mammalian cell culture wok to transiently express and purify recombinant proteins is a mustExpertise in molecular biology techniques such as PCR, DNA cloning is a mustPay Range:$88,000 - $132,000Disclosure Statement:The range provided is based on what we believe is a reasonable estimate for the base salary pay range for this job at the time of posting. This role is eligible for an annual bonus and annual equity awards. Some roles may also be eligible for overtime pay, in accordance with federal and state requirements. Actual base salary pay will be based on a number of factors, including skills, competencies, experience, and other job-related factors permitted by law.At Vertex, our Total Rewards offerings also include inclusive market-leading benefits to meet our employees wherever they are in their career, financial, family and wellbeing journey while providing flexibility and resources to support their growth and aspirations. From medical, dental and vision benefits to generous paid time off (including a week-long company shutdown in the Summer and the Winter), educational assistance programs including student loan repayment, a generous commuting subsidy, matching charitable donations, 401(k) and so much more.Flex Designation:On-Site DesignatedFlex Eligibility Status:In this On-Site designated role, you will work five days per week on-site with ad hoc flexibility.Note: The Flex status for this position is subject to Vertex's Policy on Flex @ Vertex Program and may be changed at any time.#LI-OnsiteCompany InformationVertex is a glo

Created: 2026-01-19